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Investigating Muscle Fatigue, 5.6.4 Practical: Investigating Photosynthetic Pigments with Chromatography, 5.6.6 Using the Products of the Light-Dependent Reaction, 5.6.9 Factors Affecting the Rate of Photosynthesis, 5.6.10 Practical: Investigating Factors Affecting the Rate of Photosynthesis, 5.7.3 The Four Stages in Aerobic Respiration, 5.7.10 Energy Yield of Aerobic vs Anaerobic Respiration, 5.7.11 Practical: Investigating the Rate of Respiration, 6.1.4 Gene Control: Transcription Factors, 6.1.5 Gene Control: Post-Transcriptional Modification, 6.1.7 The Importance of Mitosis & Apoptosis, 6.2.4 Predicting Inheritance: Monohybrid Crosses, 6.2.5 Predicting Inheritance: Dihybrid Crosses, 6.2.6 Predicting Inheritance: Identifying Linkage, 6.2.7 Predicting Inheritance: Identifying Epistasis, 6.2.8 Predicting Inheritance: Chi-squared Test, 6.2.9 Continuous & Discontinuous Variation, 6.4.3 Production of Artificial Clones in Plants, 6.4.6 Production of Artificial Clones in Animals, 6.4.12 Standard Growth Curve of Microorganisms, 6.4.13 Factors Affecting the Growth of Microorganisms, 6.4.14 Immobilised Enzymes in Biotechnology, 6.5.7 Testing for Distribution & Abundance, 6.6.6 Balancing Human Need & Conservation, The size of cells is typically measured using the, When doing calculations all measurements must be in the, To convert units, multiply or divide depending if the units are, There are 1000 nanometers (nm) in a micrometre (m), There are 1000 micrometres (m) in a millimetre (mm), There are 1000 millimetres (mm) in a metre (m), Magnification is how many times bigger the image of a specimen observed is in compared to the actual (real-life) size of the specimen, If two separate points cannot be resolved, they will be observed as one point, As light passes through the specimen, it will be, This means that they can be much closer before the diffracted beams overlap, The concept of resolution is why the phospholipid bilayer structure of the cell membrane cannot be observed under a light microscope, The width of the phospholipid bilayer is about 10nm, The maximum resolution of a light microscope is 200nm (half the smallest wavelength of visible light, 400nm), Any points that are separated by a distance less than 200nm (such as the 10nm phospholipid bilayer) cannot be resolved by a light microscope and therefore will not be distinguishable as separate, Light microscopes are useful for looking at, The electrons are picked up by an electromagnetic lens which then shows the image, Electron microscopes are useful for looking at, Electron microscopy requires the specimen to be. 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Step 1: Check that units in magnification questions are the same, 2000 / 1000 = 2, so the actual thickness of the leaf is 2 mm and the drawing thickness is 50 mm, Magnification = image size / actual size = 50 / 2 = 25, eyepiece lens magnification x objective lens magnification, The resolving power of an electron microscope is much greater than that of the light microscope, as structures much smaller than the wavelength of light will interfere with a beam of electrons. FREE Biology revision notes on The Microscope in Cell Studies. Lra has a particular interest in the area of infectious disease and epidemiology, and enjoys creating original educational materials that develop confidence and facilitate learning. Our worksheets cover all topics from GCSE, IGCSE and A Level courses. 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