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[4], Shewanella oneidensis MR-1 is a widely used laboratory model to study anaerobic respiration of metals and other anaerobic extracellular electron acceptors, and for teaching about microbial electrogenesis and microbial fuel cells. Before The https:// ensures that you are connecting to the Two strains of psychrotolerant bacteria, designated M7(T) and M5, isolated from Antarctic coastal marine environments were studied to determine their taxonomic position. 1992. soort herkend door Integrated Taxonomic Information System (ITIS) Taxonomic HierarchyIntegrated Taxonomic Information System (ITIS) Taxonomic Hierarchy November The discovery that OMVs can function as a lateral DNA transfer mechanism in bacteria has important implications. Bozal N, Montes MJ, Minana-Galbis D, Manresa A, Mercade E. Shewanella vesiculosa sp. Therefore, the formation of these vesicles supposes an overproduction of peptidoglycan hydrolases, and the authors proved that P. aeruginosa-derived OMVs were enriched with hydrolyzing enzymes, providing vesicles with a lytic activity against other bacteria. Images were acquired using low-dose imaging conditions and an Eagle 4k 4k charge-coupled device (CCD) camera (FEI Company, Eindhoven, Netherlands). Shewanella japonica. [2] Members of this genus are widely distributed in aquatic habitats, from the deep sea to the shallow Antarctic Ocean. Thorell K, Meier-Kolthoff JP, Sjling , Martn-Rodrguez AJ. Shewanella vesiculosa HM13 , , , , , , TEM micrographs of ultrathin sections from S. vesiculosa M7T prepared by HPF-FS. F15 - Shewanella sp. Shewanella vesiculosa M7T is a cold-adapted Antarctic bacterium that has a great capacity to secrete membrane vesicles (MVs), making it a potentially excellent model for studying the vesiculation process. 2003. Since their study, methods for analyzing bacteria with thin-section TEM have greatly improved (21). Clipboard, Search History, and several other advanced features are temporarily unavailable. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. S. vesiculosa HM13 has been found to include a cargo protein, P49, in the EMVs, but the entire mechanism in which P49 is preferentially included in the vesicles has still not been . Grids with sections were floated on 0.1 M phosphate-buffered saline (PBS) for 3 min. Shewanella is the sole genus included in the marine bacteria family Shewanellaceae.Some species within it were formerly classed as Alteromonas. TEM observations of Lowicryl HM20 thin sections of S. vesiculosa M7T OMVs also showed the presence of both types: (i) conventional or single-bilayer OMVs that were rarely marked with gold and contained non-electron-dense material (Fig. FEMS Microbiol Rev. are ubiquitous in natural environments, occurring mainly in marine environments, iced fish, proteinaceous foods, and occasionally clinical samples. Pelleted vesicles were resuspended in 25 ml of 50 mM HEPES (pH 6.8) and filtered through 0.45-m-pore-size Ultrafree spin filters (Millipore). The TEM immunolabeling technique with an antibody highly specific for dsDNA detection clearly demonstrated that the DNA quantified in S. vesiculosa M7T-derived OMVs was mostly packaged in double-layered vesicles. Expression Dynamics of Arsenic Respiration and Detoxification in Shewanella sp. Bethesda, MD 20894, Web Policies Gram-negative outer membrane vesicles: beyond the cell surface, Special delivery: vesicles trafficking in prokaryotes. Yu NY, Wagner MR, Laird G, Mellis G, Rey S, Lo R, Dao P, Sahinalp SC, Ester M, Foster LJ, Brinkman FSL. After that, grids were incubated for 30 min with a secondary goat anti-mouse antibody coupled to 12-nm colloidal gold (lot 84359; Jackson) diluted 1/20 in 1% BSA0.1 M PBS. Information on morphological and physiological properties, Information on culture and growth conditions, Information on isolation source, the sampling and environmental conditions, Information on genomic background e.g. validly published under the ICNP, Taxonomic status: LX5 - Shewanella massilia - Shewanella japonica - Shewanella sp. Federal government websites often end in .gov or .mil. Caracterizacin de las vesculas de membrana de Shewanella vesiculosa M7(T) Baeza Lara, Nicols: Discover. Proteomics in Gram-negative bacterial outer membrane vesicles, Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria. In this study, we newly isolated a cold-adapted bacterium, Shewanella vesiculosa HM13, which abundantly produces EMVs, characterized them, and analyzed their . The gram-negative bacteria Geobacter and Shewanella are two of the best-studied DMRB model systems (2, 5, 6, 10, 11) and are known to produce extracellular appendages proposed to act as bacterial nanowires, transporting electrons over micrometer-long distances to terminal extracellular EAs. 21 Glow-discharged holey carbon grids were used. Shewanella vesiculosa sp. Only proposed changes supported by the according reference will be reviewed. Sample cryoimmobilization by rapid cooling and freeze-substitution is an accepted approach to visualizing samples very close to the native structure (21) and is also one of the best methods for preserving structure under conditions compatible with immunogold labeling (22). nov., a psychrotolerant bacterium isolated from an Antarctic coastal area.Int J Syst Evol Microbiol 2009; 59:336-340. Notification that new names and new combinations have appeared in volume 59, part 2 of the IJSEM. This can be explained by the particular ability of S. vesiculosa M7T to produce these double-layered OMVs with cytoplasmic content and the capacity of the strain to form a huge amount of vesicles in general. In this study, we identified a putative sensor protein, HM1275, involved in the induction of vesicle production at high lysine concentration in a hypervesiculating Gram-negative bacterium, Shewanella vesiculosa HM13. The type strain is M7(T) (=LMG 24424(T) =CECT 7339(T)). facultative anaerobe. The subcellular localization of some proteins, mainly hypothetical, was unknown (U) (13%). Even for the conventional OMVs described to date, it has proved extremely difficult to visualize vesicles at this stage of formation, and practically no quality images are available in the numerous published reports (8, 15, 20, 28). Shewanella vesiculosa: KDH10_003830: Help: Entry: KDH10_003830 CDS T08322 : Name (GenBank) WD40 repeat domain-containing protein. The excess liquid was blotted with filter paper, leaving thin sample films spanning the grid holes. nov., a novel moderately halophilic bacterium from an Antarctic environment. 2005 May;11(5):347-52. doi: 10.1111/j.1469-0691.2005.01108.x. The putative functions and subcellular localization of the proteins identified in OMVs from S. vesiculosa M7T are summarized in Table S1 in the supplemental material. Some species within it were formerly classed as Alteromonas. Grids were rinsed with double-distilled water and dried with filter paper. The two prominent bands observed in 1-D SDS-polyacrylamide gels belonged to this category (Fig. In addition, a proteomic study of purified membrane vesicles confirmed the presence of plasma membrane and cytoplasmic proteins in OMVs from this strain. If your changes are legitimate, changes will occur within the next BacDive update. List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. Plasma membrane and cytoplasmic content are included in the vesicle that is leaving the cell, and DNA can thus be incorporated. On the basis of the data from this polyphasic taxonomic study, M7(T) and M5 constitute a single genospecies. Data source record id: GCF_021560015.1 (NCBI assembly accession): Data source organism name: Shewanella vesiculosa: NCBI BioSample: SAMN18806533: NCBI BioProject . nov., isolated from marine fish of the Baltic Sea. Shewanella fidelis Ivanova et al. Lee EY, Choi DS, Kwang-Pyo K, Yong SG. Accessibility Bars, 100 nm (A, C, E) and 200 nm (B, D, F). Several phenotypic characteristics, the cellular fatty acid compositions and the quinone content of strains M7(T) and M5 served to differentiate them from related shewanellae. In addition, the OMVs were surrounded by the same fringe of fine fibers as the cells and contained a low-electron-density similar material to that in the periplasmic space of the cells. BR - Shewanella vesiculosa - Shewanella sp. OIMVs with a double-bilayer structure have also been identified in ExPEC EVs with TEM. (C) TEM micrograph showing an isolated double-bilayer vesicle from this strain after immunolabeling with a dsDNA antibody. 2019 Aug 28;10:1861. doi: 10.3389/fmicb.2019.01861. Third, the grids were preincubated at 37C for 6 h with 1 mg/ml DNase I (Sigma) in PBS plus 7 mM MgCl2, and then the grids were thoroughly washed with water before immunolabeling with dsDNA monoclonal antibody. Exuding a sophisticated elegance, Mandarin Oriental, Taipei is the preferred residence for discerning international travellers and local elites. 8600 Rockville Pike Int J Syst Evol Microbiol. For the TEM observation of S. vesiculosa M7T cells, electron microscopy analysis of Epon-embedded thin sections was performed as described by Frias et al. Analyse 2008. Carla Prez-Cruz is the recipient of fellowship FFAR2012.3 from the University of Barcelona. 2. Peptides were separated by liquid chromatography and subsequently analyzed using a nano-ESI-QTOF mass spectrometer. doi: 10.1093/femsre/fuaa047. (D) TEM micrograph of OMVs labeled with a primary IgM monoclonal antibody to Plasmodium falciparum with no affinity to DNA and a secondary antibody coupled to 12-nm colloidal gold. It was remarkable that when observed in TEM sections, several of the observed double-bilayer vesicles showed an elongated shape at the time of formation and expulsion from the cell, but once liberated, vesicles became almost round. Shewanella vesiculosa M7 T is a cold-adapted Antarctic bacterium that has a great capacity to secrete membrane vesicles (MVs), making it a potentially excellent model for studying the vesiculation process. The presence of DNA inside bacterial OMVs and the possibility that these structures constitute a new mechanism of lateral gene transfer have important implications in several areas, including prokaryotic evolution and in particular the transfer of antibiotic resistance genes or virulence genes within bacteria (11, 12, 13, 14, 15, 16, 17, 18, 19). 2011. 2009. National Library of Medicine Lee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS. Strain M5 showed 100 % DNA relatedness with respect to strain M7(T). As expected, the identified vesicular proteins were mainly from the outer membrane (OM) (69.57%), with most of them being TonB-dependent receptors and porins involved in inorganic ion transport and metabolism. The genome sequence for this bacterium is not available, and proteins were putatively identified by their similarity to proteins from related species. The https:// ensures that you are connecting to the Received 2012 Nov 26; Accepted 2013 Jan 5. S. vesiculosa HM13 has been found to include a cargo protein . No gold mark is observed. Supplemental material for this article may be found at http://dx.doi.org/10.1128/AEM.03657-12. 2009, Etymology: Shewanella GC3546 Shewanella vesiculosa. Bar, 2 % sequence divergence. In recent years, many studies have been conducted on outer membrane vesicles (OMVs) produced by Gram-negative bacteria (1). The presence of double-bilayer OMVs was also confirmed by cryo-TEM of thin frozen films of isolated OMVs. motile. Protein bands (Fig. This study was supported by the Government of Spain (CICYT project CTQ 2010-21183-C02-01/PPQ) and by the Autonomous Government of Catalonia (grant 2009SGR1212;). The DNA content of OMVs was 2.1 0.4 ng DNA/g OMV protein before DNase treatment and 1.8 0.24 ng DNA/g OMV protein afterwards. Bacterial extracellular membrane vesicles (EMVs) play an active role in many physiological and pathogenic processes. [5], Colony, morphological, physiological, and biochemical characteristics of Shewanella species are shown in the Table below. N.L. Frias A, Manresa A, de Oliveira E, Lopez-Iglesias C, Mercad E. (E) TEM micrograph of OMVs from grids preincubated with 1 mg/ml DNase I and then immunolabeled with a dsDNA IgM and a secondary antibody coupled to gold. Vesicles were collected from exponentially growing cultures to avoid the presence of lysed cells. Shewanella vesiculosa M7T is a cold-adapted Antarctic bacterium that has a great capacity to secrete membrane vesicles (MVs), making it a potentially excellent model for studying the vesiculation . The subcellular localization of proteins was analyzed using the PSORTb v3.0.2 program (24). Vesicles were further purified for proteomic analysis using a method adapted from that described by Horstman and Kuehn (3). Semantic Scholar uses AI to extract papers important to this topic. The .gov means its official. about navigating our updated article layout. Federal government websites often end in .gov or .mil. ve.si.cu.losa. Located in Northern Taiwan, Taipei City is an enclave of the municipality of New Taipei City that sits about 25 km (16 mi) southwest of the northern port city of Keelung.Most of the city rests on the Taipei Basin, an ancient lakebed. Int J Syst Evol Microbiol 2009; 59:336-340. The images we obtained of double-bilayer OMVs of S. vesiculosa M7T corroborate one of the models proposed by Kadurugamuwa and Beveridge (4) to explain how certain macromolecules in Pseudomonas aeruginosa, such as cytoplasmic enzymes and DNA, are exported via membrane vesicles. C31 - Shewanella sp. This strain can grow at temperatures ranging from 4C to 34C, and one of its prominent traits is an ability to produce a huge amount of natural OMVs from solid or liquid cultures without any inducing factors such as the addition of membrane-perturbing agents. Name: Shewanella MacDonell and Colwell 1986 Category: Genus Proposed as: gen. nov. Etymology: She.wan.el'la. (23). However, how bacteria regulate vesiculation in response to the extracellular environment remains largely unknown. The temperature was secreted by five cold-adapted Antarctic bacteria: Shewanella gradually increased (t=5C/h) to 4C and held constant for T T livingstonensis NF22 , Shewanella vesiculosa M7 , 2 h, and then finally increased to room temperature and Pseudoalteromonas sp. Linking: This result confirmed that most DNA was inside the vesicles and not surface associated, since approximately 85% remained after DNase treatment. Unexpectedly, however, in some S. vesiculosa M7T cells we noted the formation of membrane vesicles in which both the outer membrane (OM) and plasma membrane (PM) were extruded. Shewanella vesiculosa M7T is an Antarctic psychrotolerant Gram-negative bacterium isolated by our research group from marine sediments collected on Deception Island (South Shetland Islands) (25). In one model, extracellular DNA released after bacterial lysis is internalized in the vesicles by a mechanism similar to that used in bacterial transformation. In Fig. Moreover, double-bilayer vesicles isolated from liquid media were also observed using the cryo-TEM technique, in which the specimen was undoubtedly native and free of chemical artifacts. National Institutes of Health Create Alert Alert. They represent a novel species of the genus Shewanella, for which the name Shewanella vesiculosa sp. The cells were pelleted by centrifugation at 10,000 g for 10 min at 4C, and the supernatant was filtered through 0.45-m-pore-size filters to remove remaining bacterial cells. [11], The discovery of some of the respiratory capabilities possessed by members of this genus has opened the door to possible applications for these bacteria. 8600 Rockville Pike 1990 emend. (B) TEM micrograph showing double-bilayer OMVs immunolabeled like the vesicles in panel A. C27 - Shewanella sp. [15] Its ability to decrease toxicity of various substances makes Shewanella a useful tool for bioremediation. We provide comprehensive resources for bacterial identification, genomics and microbiome. Previous structural studies with this strain allowed us to observe that this Gram-negative bacterium produces different types of OMVs, including conventional vesicles with a single bilayer and another, more complex type with a double bilayer and containing electron-dense material. Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. 3C). FOIA OM, outer membrane; PM, plasma membrane; OMV, outer membrane vesicle; HPF-FS, high-pressure freezing and freeze-substitution. An ultrastructural study of the Antarctic psychrotolerant bacterium Shewanella vesiculosa M7T has revealed that this Gram-negative bacterium naturally releases conventional one-bilayer OMVs through a process in which the outer membrane is exfoliated and only the periplasm is entrapped, together with a more complex type of OMV, previously undescribed, which on formation drag along inner membrane and cytoplasmic content and can therefore also entrap DNA. nov., isolated from the gut microflora of abalone, Haliotis discus hannai", List of bacterial genera named after personal names, Comparative Analysis of Shewanella Genomes, https://en.wikipedia.org/w/index.php?title=Shewanella&oldid=1076572071, This page was last edited on 11 March 2022, at 19:54. A double-bilayer vesicle can be observed with an inner layer that corresponds to the plasma membrane (PM) and an outer layer corresponding to the outer membrane (OM). Vesicle size was determined with the program analySIS (Soft Imagine System, Switzerland). Samples were then washed for 3.5 h in methanol at 50C and infiltrated in a graded series of HM20-methanol mixtures: 1:3 for 3 h, 2:2 overnight, 3:1 for 6 h, and 3 changes of pure HM20 (Electron Microscopy Sciences) for 48 h. Samples were polymerized at 50C for 24 h by indirect UV irradiation (360 nm), followed by further hardening at room temperature for 48 h. Ultrathin sections were obtained as described above. Multifunctional membrane vesicles in Pseudomonas aeruginosa, Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. The latter showed an external bilayer membrane that corresponded to the cell OM and an inner membrane also showing a bilayer structure that corresponded to the cell PM, as depicted in Fig. PMC legacy view [13], One of the roles that the genus Shewanella has in the environment is bioremediation. Learn more Briefly, proteins were in-gel digested with trypsin, and then tryptic peptides were extracted from the gel matrix, pooled, and dried in a vacuum centrifuge. Finally, when grids with sections were preincubated at 37C for 6 h with 1 mg/ml DNase and then immunolabeled with the anti-DNA antibody, gold markers were not detected, showing that the DNA within double-bilayer vesicles was degraded by DNase treatment (Fig. [ n. Shewanella, named after James Shewan for his work in fisheries microbiology Gender: feminine (stem: Shewanell-) Type species: Shewanella putrefaciens (Lee et al. The new double-bilayer OMVs were estimated by cryo-TEM to represent 0.1% of total vesicles. [8] Marine species have demonstrated an ability to use arsenic as an electron acceptor as well. Reduction of iron and manganese through Shewanella respiration has been shown to involve extracellular electron transfer through the employment of bacterial nanowires, extensions of the outer membrane. [3] Shewanella chilikensis, a species of the genus Shewanella commonly found in the marine sponges of Saint Martin's Island of the Bay of Bengal, Bangladesh. 1981) MacDonell and Colwell 1986 Conduct genome-based taxonomy of genus at TYGS Shewanella chilikensis sp. We should also point out that no opened vesicles were observed in the cryo-TEM analysis of OMVs from S. vesiculosa M7T. As of 2020-09-20 this emendation was not yet included in an IJSEM List of Changes in Taxonomic Opinion. An ultrastructural study of the Antarctic psychrotolerant bacterium Shewanella vesiculosa M7(T) has revealed that this Gram-negative bacterium naturally releases conventional one-bilayer OMVs .
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